Allele/Variant

Tg(Hbb-b1)83Clo

Species
Mus musculus
Symbol
Tg(Hbb-b1)83Clo
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • TgN(Hbb-b1)83Clo
  • TgN(Hbb-b1)83Lo
Description
The transgenic construct comprised a pBR322-derived plasmid containing a 7.0-kb mouse genomic EcoRI fragment that included the mouse beta-major globin gene. In situ hybridization of a plasmid-derived probe to bone marrow metaphase chromosome spreads localized the integration site to the telomeric end of Chr 3. 800-1000 tandem copies of the transgene were estimated, by Southern blot analysis, to have integrated in this transgenic line. Northern blot analysis confirmed the expected lack (due to presence of the pBR322 sequences) of transcription of the exogenous globin genes, and analysis of blood from mice bearing the transgene detected no expression of the beta-major globin chain in their hemoglobin.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
Knock-down Targets
None
Regulatory Regions
None
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations