Allele/Variant

Tg(Tek-Jam3)1Maal

Species
Mus musculus
Symbol
Tg(Tek-Jam3)1Maal
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • pHHNS-JAM-C
  • Tg(Tie2-Jam-C)1Maal
Description
The transgene contains the coding sequence, 3' untranslated region and polyadenylation signal of mouse junction adhesion molecule 3 (Jam3) inserted near the 3' end of the first exon of the mouse endothelial-specific receptor tyrosine kinase gene (Tek) in a genomic DNA fragment containing 2.1 kb of upstream DNA containing Tek promoter sequences, most of exon 1 and, following the inserted Jam3 sequence, ~10 kb of the first half of intron 1, which contains endothelial cell-specific enhancer elements.

Western blot analysis of total lung lysates and of immunoprecipitates of lung lysates demonstrate elevated expression of JAM3 in lungs of transgenic mice versus control littermates. Flow cytometric analysis of dissociated lung tissue cells stained for JAM3, the endothelial-cell marker VE-cadherin and the hematopoietic cell marker CD45 show 3-fold enhanced expression of JAM3 specifically on endothelial cells.

Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
Knock-down Targets
None
Regulatory Regions
None
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations