Allele/Variant

Sqstm1tm1Jjw

Species
Mus musculus
Symbol
Sqstm1tm1Jjw
Category
allele
Allele of gene
Sqstm1
Transgenic Constructs
None
Synonyms
  • p62P394L
  • p62KI
Description
A targeting vector was constructed to engineer a point mutation into the gene locus. A P394L mutation (a C-to-T transition) was introduced into exon 8 by PCR-based site-directed mutagenesis. This mutation is equivalent to the P392L substitution in the human gene. A loxP-flanked neo cassette was also introduced immediately downstream of exon 7. After targeting, transient cre-recombinase expression removed the neo cassette leaving a single loxP site behind. Southern blot and genomic PCR analysis confirmed correct targeting of the construct.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations