Allele/Variant

Tg(Myh6-Pln)22Egk

Species
Mus musculus
Symbol
Tg(Myh6-Pln)22Egk
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • T17A mutant PLB
  • T17A PLB
Description
The transgene contains a full length mouse cardiac phospholamban cDNA with a point mutation (ACT->GCT) that encodes alanine instead of threonine at amino acid position 17 of the protein (T17A), followed by an SV40 polyadenylation signal, under control of the cardiac specific alpha myosin heavy chain (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The replaced threonine normally is phosphorylated in response to stimulation of cardiac muscle beta adrenergic receptors. Northern blot analysis revealed cardiac expression of the 1.0 kb transcript from the transgene, and quantitative immunoblot analysis demonstrated expression of phospholamban protein in the hearts of transgenic mice homozygous for a null phospholamban allele.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
Knock-down Targets
None
Regulatory Regions
None
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations