Allele/Variant

Gata4Tg(Tyr)2265AOve

Species
Mus musculus
Symbol
Gata4Tg(Tyr)2265AOve
Category
allele
Allele of gene
Gata4
Transgenic Constructs
None
Synonyms
  • OVE#2265A
  • OVE2265A
Description
A lentiviral transgenic approach was used to generate these mice. The Tyro-WPRE-FUGW lentiviral transgene (LV2177) was designed with a mouse tyrosinase minigene (Tyro) and woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2265A, the lentivirus integrates into intron 2 [NCBI37/mm9; 3'-63,825,313(+)] in the antisense orientation.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations