Allele/Variant

Ins1em1(cre)Utr

Species
Mus musculus
Symbol
Ins1em1(cre)Utr
Category
allele
Allele of gene
Ins1
Transgenic Constructs
Synonyms
None
Description
The bicistronic Ins1-2A-Cre knock-in allele was created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (cre) just upstream of the endogenous STOP codon of Ins1. An annealed Ins1-CRISPR-F and Ins1-CRISPR-R were inserted into the entry site of plasmid carrying both gRNA and Cas9 expression units. DNA vectors were microinjected into the pronuclei of C57BL/6J fertilized zygotes. Embryos were transferred to pseudopregnant females.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
None
Knock-down Targets
None
Regulatory Regions
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

Phenotype
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Source
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    Disease Associations

    Association
    Disease Qualifier
    Disease
    Annotation details
    Evidence
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