Predicted to enable alpha-mannosidase activity. Predicted to be involved in N-glycan processing. Located in Golgi apparatus. Is expressed in head; hypodermis; intestine; nervous system; and pharynx. Orthologous to several human genes including MAN2A2 (mannosidase alpha class 2A member 2).
WB Description
aman-2 encodes an alpha-mannosidase II, homologous to human MAN2A1(OMIM:154582) and MAN2A2, that is required for normal N-glycanstructures and predicted to act in the Golgi; AMAN-2 is predicted tomature glycoproteins by removing mannose residues from their N-linkedoligosaccharides; translational fusions of AMAN-2 with GFP aresubcellularly localized in a pattern consistent with Golgi function;aman-2 is broadly expressed in larvae; in adults, aman-2 is moststrongly expressed in the digestive system (gut wall, pharynx andgrinder), hypodermal cells, and neurons; aman-2 mutants have N-glycansnot seen in normal worms, such as Hex5-7HexNAc2-3Fuc2Me, along withhybrid oligosaccharides, while having reduced paucimannosidic andtrifucosylated glycans, and completely lacking tetrafucosylated ones;AMAN-2 contains three predicted N-glycosylation sites, one of which(Asn-320) is known to be occupied in vivo; Cu(II) was observed tocompletely inhibit AMAN-2, which it also does with mouse and Drosophilaalpha-mannosidase II; the pH optimum of AMAN-2 is pH 5.5-6.0; severalother ionic conditions (e.g., Co[II] or EDTA) have little effect onAMAN-2; aman-2(tm1078) mutants are superficially wild-type, but mutantextracts completely lack alpha-mannosidase II activity; aman-2(tm1078)mutants have reduced, but not absent, anti-horseradish peroxidase andphosphorylcholine epitopes.