Gene

aman-2

Species
Caenorhabditis elegans
Symbol
aman-2
Name
Alpha MANnosidase 2
Synonyms
  • CELE_F58H1.1
  • F58H1.1
Biotype
protein coding gene
Automated Description
Predicted to enable alpha-mannosidase activity. Predicted to be involved in N-glycan processing. Located in Golgi apparatus. Is expressed in head; hypodermis; intestine; nervous system; and pharynx. Orthologous to several human genes including MAN2A2 (mannosidase alpha class 2A member 2).
WB Description
aman-2 encodes an alpha-mannosidase II, homologous to human MAN2A1(OMIM:154582) and MAN2A2, that is required for normal N-glycanstructures and predicted to act in the Golgi; AMAN-2 is predicted tomature glycoproteins by removing mannose residues from their N-linkedoligosaccharides; translational fusions of AMAN-2 with GFP aresubcellularly localized in a pattern consistent with Golgi function;aman-2 is broadly expressed in larvae; in adults, aman-2 is moststrongly expressed in the digestive system (gut wall, pharynx andgrinder), hypodermal cells, and neurons; aman-2 mutants have N-glycansnot seen in normal worms, such as Hex5-7HexNAc2-3Fuc2Me, along withhybrid oligosaccharides, while having reduced paucimannosidic andtrifucosylated glycans, and completely lacking tetrafucosylated ones;AMAN-2 contains three predicted N-glycosylation sites, one of which(Asn-320) is known to be occupied in vivo; Cu(II) was observed tocompletely inhibit AMAN-2, which it also does with mouse and Drosophilaalpha-mannosidase II; the pH optimum of AMAN-2 is pH 5.5-6.0; severalother ionic conditions (e.g., Co[II] or EDTA) have little effect onAMAN-2; aman-2(tm1078) mutants are superficially wild-type, but mutantextracts completely lack alpha-mannosidase II activity; aman-2(tm1078)mutants have reduced, but not absent, anti-horseradish peroxidase andphosphorylcholine epitopes.
Cross References
Additional Information
Literature

Orthology

Gene tree
PANTHER:PTHR11607
Links to orthology data in JBrowse by filter level: Stringent,  Moderate,  No filter,  Best and Best Reverse

Paralogy

Function - GO Annotations

Pathways

No data available

Phenotypes

Primary Sources
Other Sources
None

Disease Associations

Cases where the expected disease association was NOT found
Cell color indicative of annotation volume

Transgenic Alleles

Models

Sequence Feature Viewer

Genome location
Assembly version
WBcel235
Viewer Help
11.9280M11.9285M11.9290M11.9295M11.9300M11.9305M11.9310M11.9315M11.9320M11.9325M11.9330M

Sequence Details

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Expression

Primary Sources
Other Sources
Cell color indicative of annotation volume; red slash indicates species lacks structure or developmental stage.

Molecular Interactions

Genetic Interactions