Predicted to enable DNA binding activity and endonuclease activity. Predicted to contribute to crossover junction DNA endonuclease activity. Predicted to be involved in DNA metabolic process and mitotic intra-S DNA damage checkpoint signaling. Predicted to act upstream of or within DNA recombination and DNA repair. Predicted to be located in nucleus. Predicted to be part of Holliday junction resolvase complex. Orthologous to human EME1 (essential meiotic structure-specific endonuclease 1).