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Summary: Emx2Cre/Cre vs. Emx2WT/WT by RNAseq 10 utricles were dissected and pooled for each technical replicate and stored in RNAlater at 4C until genotypes were verified. RNA extraction was completed via Qiagen RNeasy micro Kit with DNAse I column digest for 4 technical replicates of each genotype. Concentrations were validated with Qubit and quality scores using Agilent High Sensitivity RNA ScreenTape Assay prior to RNA analysis. RNA library prep was completed using Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold kit. Sequencing was completed with NovaSeq2 x 150bp Sequencing 200M read-pairs per sample. Subsequent alignments identified human RNA transcript read contamination in a single replicate, which was removed prior to DESeq2 analysis to identify upregulated and downregulated transcripts.

Symbol: Emx2 KO RNAseq

Name: Emx2 KO RNAseq

Expression data from Emx2 wildtype and knockout olfactory epithelium

(Mus musculus)

HTP Dataset Index

High-Throughput (HTP) Dataset Index metadata provided by MGI

ID: ArrayExpress:E-GEOD-12135, GEO:GSE12135

Tags: WT vs. mutant, genotype

Summary: Emx2 is a homeobox transcription factor that plays a critical role in development. Olfactory sensory neuron axons from Emx2 knockout mice fail to innervate their target tissue, the olfactory bulb. Homeobox transcription factors may also play an important role in olfactory receptor expression. We used microarrays to analyze differences in mRNA abundance in the olfactory epithelium of Emx2 wildtype and knockout embryonic day 18.5 mice. Keywords: Knockout-Wildtype comparision Total RNA from 9 Emx2 wildtype and 9 Emx2 knockout mice was analyzed. Equal amounts of RNA from 3 animals was pooled to create one biological sample, 3 pools per genotype, 6 chips total. RNA was hybridized to the Affymetrix Mouse Exon 1.0 ST array.

Symbol: Expression data from Emx2 wildtype and knockout olfactory epithelium

Name: Expression data from Emx2 wildtype and knockout olfactory epithelium

Transcription profiling of mouse Emx2 knock-out urogenital epithelium

(Mus musculus)

HTP Dataset Index

High-Throughput (HTP) Dataset Index metadata provided by MGI

ID: ArrayExpress:E-GEOD-10216, GEO:GSE10216

Tags: WT vs. mutant, genotype

Summary: Series of samples studying effect of knock out Emx2 in urogenital epithelium of mouse embryos at E10.5. Experiment Overall Design: The epithelial cells of the gonadal primordium were obtained by Laser Microdissection System. The specimens prepared from three individuals were mixed as one pool. There are three experimental replicates in each genotype, 3-pools of wild-type and 3-pools of Emx2 KO mouse.

Symbol: Transcription profiling of mouse Emx2 knock-out urogenital epithelium

Name: Transcription profiling of mouse Emx2 knock-out urogenital epithelium

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