Oatp74D^{CR00591-TG4.2}

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Species

Drosophila melanogaster

Symbol

Oatp74D^{CR00591-TG4.2}

Category

allele

Allele of gene

Oatp74D

Transgenic Constructs

None

Synonyms

• Oatp74D[CR00591-TG4.2]

Description

A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Oatp74D, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TTGCATACTTATGAGGGCAGTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.2} cassette is predicted to be accompanied by a deletion of 3bp of genomic sequence flanking the insertion site. The PCR check of the insertion gave the expected sized product on the right flank and a shorter than expected sized product for the left flank.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations