asRNA:CR45108^{CR00701-TG4.0-X}

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Species

Drosophila melanogaster

Symbol

asRNA:CR45108^{CR00701-TG4.0-X}

Category

allele

Allele of gene

asRNA:CR45108

Transgenic Constructs

None

Synonyms

• asRNA:CR45108[CR00701-TG4.0-X]

Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into cn, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion is also within asRNA:CR45108, not in a coding intron, and is not predicted to gene trap this gene. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: AATCCCTGCTTCCAGATATAAGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.0} cassette is predicted to be accompanied by a deletion of 10bp of genomic sequence flanking the insertion site. The PCR check of the insertion gave the expected sized product for the left flank, but no product for the right flank.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations