Ir76a^{CR60096-TG4.1-X}

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Species

Drosophila melanogaster

Symbol

Ir76a^{CR60096-TG4.1-X}

Category

allele

Allele of gene

Ir76a

Transgenic Constructs

None

Synonyms

• Ir76a[CR60096-TG4.1-X]

Description

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into CG14102, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion is also within Ir76a, in a coding intron, and is not predicted to gene trap this gene. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CACCGACTCGAATGAAGTTTAGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 1bp of genomic sequence flanking the insertion site.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations