CG31915^{CR70562-KO-kG4}

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Species

Drosophila melanogaster

Symbol

CG31915^{CR70562-KO-kG4}

Category

allele

Allele of gene

CG31915

Transgenic Constructs

None

Synonyms

• CG31915[CR70562-KO-kG4]

Description

A TI{KozakGAL4} DNA cassette has been inserted into CG31915, replacing the coding sequence (coordinates of deleted sequence are 2L:5528148..5530226, release 6 genome). This results in a simultaneous knock-out of CG31915 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of CG31915 (predicted to gene trap all annotated transcripts of the gene). The deletion also removes part of Cap-D3 (intronic sequence as CG31915 is nested within an intron of Cap-D3). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ATGCGCCGGAGAATCAGATTCGG and AGGCACATTTGGCAGTTGTTAGG.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations