Allele/Variant

CG9231CR70651-KO-kG4

Species
Drosophila melanogaster
Symbol
CG9231CR70651-KO-kG4
Category
allele
Allele of gene
CG9231
Transgenic Constructs
None
Synonyms
  • CG9231[CR70651-KO-kG4]
Description
A TI{KozakGAL4} DNA cassette has been inserted into CG9231, replacing the coding sequence (coordinates of deleted sequence are 3L:19585441..19585926, release 6 genome). This results in a simultaneous knock-out of CG9231 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of CG9231 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ACTTGTGAAACGATTAAAAAAGG and TGGCCAGTTCTAATATTACTAGG.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

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    Disease Associations

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