Gene

bro-1

Species
Caenorhabditis elegans
Symbol
bro-1
Name
BROther (Drosophila tx factor partner) homolog 1
Synonyms
  • CELE_F56A3.5
  • F56A3.5
Biotype
protein coding gene
Automated Description
Enables transcription corepressor activity. Involved in several processes, including negative regulation of transcription by RNA polymerase II; nematode male tail tip morphogenesis; and positive regulation of locomotion involved in locomotory behavior. Located in nucleus. Part of core-binding activity factor complex. Is expressed in several structures, including hypodermis; intestine; pharyngeal neurons; ray precursor cell; and uterine seam cell. Human ortholog(s) of this gene implicated in acute myelomonocytic leukemia. Orthologous to human CBFB (core-binding factor subunit beta).
WB Description
bro-1 encodes an ortholog of human CBFB (OMIM:121360, mutated in acute myeloid leukemia and CBFB haploinsufficiency) and Drosophila BRO/BGB; BRO-1 is required for the normal proliferation and differentiation of seam cells, alae, and male tail rays; bro-1 mutants exhibit defective seam cell division and differentiation, and males exhibit ray loss; conversely, transgenic overexpression of BRO-1 induces excess seam cell divisions at least somewhat independently of RNT-1; coexpression of both BRO-1 and RNT-1 induces full seam cell hyperplasia; rnt-1 transcription is abnormally high in bro-1 mutant larvae; BRO-1 is also required for normal structural integrity of the vulva, with bro-1 mutants showing ruptured vulvae; BRO-1 is expressed in both cytoplasm and nuclei of hypodermal seam cells (from bean-stage embryos onward), muscle cells, the uterine seam (utse) cell in late L4 larvae, some pharyngeal neurons, and male ray precursor cells, and transgenic expression of BRO-1 in seam cells alone rescues the bro-1(bp133) mutation; seam cell phenotypes of bro-1 mutants are partly suppressed by inactivation of LIN-35, FZR-1, and CKI-1, all of which inhibit progression from G1 to S phase of the cell cycle; the structural similarity of BRO-1 to CBFbeta has been confirmed by circular dichroism; BRO-1 is bound in vitro by the RUNT domains (RDs) of either RNT-1 or its human ortholog RUNX1; although BRO-1 does not itself bind DNA, it does bind to a complex of DNA with RNT-1's RD, and enhances both the strength and the specificity of RNT-1's DNA binding when it does so; BRO-1 is genetically redundant with UNC-37 and SOP-1 in specifying seam cell fate, and BRO-1's ligand RNT-1 binds UNC-37 in vitro; bro-1(bp133) and sop-1(RNAi) also show synthetic lethality.
Cross References
Additional Information
Literature

Orthology

Gene tree
PANTHER:PTHR10276
Links to orthology data in JBrowse by filter level: Stringent,  Moderate,  No filter,  Best and Best Reverse

Paralogy

No paralogs for the gene.

Function - GO Annotations

Pathways

No data available

Phenotypes

Primary Sources
Other Sources
None

Disease Associations

Cases where the expected disease association was NOT found
Must provide at least one subject
Cell color indicative of annotation volume

Transgenic Alleles

Models

No data available

Sequence Feature Viewer

Genome location
Assembly version
WBcel235
Viewer Help
5.1870M5.1872M5.1874M5.1876M5.1878M5.1880M5.1882M5.1884M5.1886M5.1888M5.1890M5.1892M

Sequence Details

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Expression

Primary Sources
Other Sources
Must provide at least one subject
Cell color indicative of annotation volume; red slash indicates species lacks structure or developmental stage.

Molecular Interactions

7 interactor genes based on 9 annotations
bro-1 molecule type
Interactor gene
Interactor species
Interactor molecule type
Detection methods
Source
Reference
DNA
cec-7Caenorhabditis elegans
protein
  • one hybrid
PMID:27777270
DNA
nhr-16Caenorhabditis elegans
protein
  • one hybrid
PMID:27777270
DNA
nhr-20Caenorhabditis elegans
protein
  • one hybrid
PMID:27777270
DNA
nhr-86Caenorhabditis elegans
protein
  • one hybrid
PMID:27777270
protein
rnt-1Caenorhabditis elegans
protein
  • affinity chromatography technology
PMID:17706957
protein
rnt-1Caenorhabditis elegans
protein
  • enzymatic study
PMID:17933794
protein
rnt-1Caenorhabditis elegans
protein
  • far western blotting
PMID:17206850
Showing 1 - 7 of 7 rows
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Genetic Interactions

bro-1 role
bro-1 genetic perturbation
Interactor gene
Interactor species
Interactor role
Interactor genetic perturbation
Interaction type
Phenotype or trait
Source
Reference
enhancer gene
tm1183nhr-25Caenorhabditis elegans
enhanced gene
genetic enhancement
    PMID:19654209
    suppressor gene
    tm1183pop-1Caenorhabditis elegans
    suppressed gene
    genetic suppression
      PMID:20849842
      Showing 1 - 2 of 2 rows
      per page