Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
shits1.IVS.Syn21.p10.20xUAS.GFP
- Species
- Drosophila melanogaster
- Symbol
- shits1.IVS.Syn21.p10.20xUAS.GFP
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- shi[ts1.IVS.Syn21.p10.20xScer\UAS.T:Avic\GFP]
- shi[ts1.IVS.Syn21.p10.20xUAS.GFP]
- Description
- 20 copies of the UAS site are fused upstream of the shi1 coding region (this encodes a temperature-sensitive dominant negative form of shi) which is tagged at the C-terminal end with GFP. A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and coding sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the coding sequence.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 2 transgenic constructs- Symbol
- P{20XUAS-IVS-Syn21-shi
-GFP-p10} - Expressed Components
- shi (Dme)
- GFP
- Knock-down Targets
- None
- Regulatory Regions
- UAS
- Symbol
- PBac{20XUAS-IVS-Syn21-shi
-GFP-p10} - Expressed Components
- shi (Dme)
- GFP
- Knock-down Targets
- None
- Regulatory Regions
- UAS