Syt14^{CR00553-TG4.0}

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Species

Drosophila melanogaster

Symbol

Syt14^{CR00553-TG4.0}

Category

allele

Allele of gene

Syt14

Transgenic Constructs

None

Synonyms

• Syt14[CR00553-TG4.0]

Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into Syt14, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: AAAGGCATTCAACTTTCGGTCGG. The homology arms of the donor vector overlapped by 8 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations