A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Hlc, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion is also within snoRNA:MeU6-A47, not in a coding intron, and it is not clear whether or not it acts to gene trap this gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: GCAACTAATCTTCTCGCTGACGG.