Gat^{CR01191-TG4.0}

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Species

Drosophila melanogaster

Symbol

Gat^{CR01191-TG4.0}

Category

allele

Allele of gene

Gat

Transgenic Constructs

None

Synonyms

• Gat[CR01191-TG4.0]

Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into Gat, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TTCATCCCTCATCTAGTTGTTGG. The homology arms of the donor plasmid overlapped by 9bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA. The PCR check of the insertion gave a shorter than expected band for the right flank and the expected sized product for the left flank.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations