Scer\GAL4^{Wbp2-CR01871-TG4.2}

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Species

Drosophila melanogaster

Symbol

Scer\GAL4^{Wbp2-CR01871-TG4.2}

Category

allele

Allele of gene

None

Transgenic Constructs

• TI{CRIMIC.TG4.2}

Synonyms

• Scer\GAL4[Wbp2-CR01871-TG4.2]

Description

A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Wbp2, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TAAATGTAAACACTGACGTGTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.2} cassette is predicted to be accompanied by a deletion of 24bp of genomic sequence flanking the insertion site.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations