Gat^{CR01191-DH.PT-GFSTF.0}

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Species

Drosophila melanogaster

Symbol

Gat^{CR01191-DH.PT-GFSTF.0}

Category

allele

Allele of gene

Gat

Transgenic Constructs

None

Synonyms

• Gat[CR01191-DH.PT-GFSTF.0]

Description

The RMCE cassette of the TI{CRIMIC.TG4.0}Gat^{CR01191-TG4.0} insertion has been replaced with a 'Double Header' (DH) trapping cassette (contains two independent trapping modules oriented in opposite directions). The insertion is in a coding intron of Gat (in the protein-trap orientation relative to the gene) and is predicted to trap the gene with a EGFP-FlAsH-StrepII-TEV-3xFlag multi-tag cassette. The homology arms of the donor vector overlapped by 9 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA. The PCR check of the insertion gave a shorter than expected band for the right flank and the expected sized product for the left flank.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations