Sell^tm1Hyn

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Species

Mus musculus

Symbol

Sell^tm1Hyn

Category

allele

Allele of gene

Sell

Transgenic Constructs

None

Synonyms

• L-Sel^-
• L^-

Description

The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. In order to create a triple knockout, ES cells heterozygote for the Selp^tm1Hyn and Sele^tm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of all three encoded proteins in leukocytes isolated from homozygotes. This targeted mutation occurred in cis with the other two null mutations. Due to their genomic proximity, the three null alleles should propagate together through the offspring.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations