Cd3e^tm1Mal

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Species

Mus musculus

Symbol

Cd3e^tm1Mal

Category

allele

Allele of gene

Cd3e

Transgenic Constructs

None

Synonyms

• Cd3-e^delta5
• Cd3-e^delta55

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Description

A PGK-neomycin cassette replaced most of exon 5, which encodes the extracellular domain. Northern analysis detected a faint, aberrant ~1.3kb transcript in thymocytes of homozygous mutant mice. PCR studies of cDNA synthesized from total thymocyte RNA did not detect wild-type mRNA in homozygous mutant mice using an internal, exon 8 oligo probe. However, these studies did detect a faint truncated transcript in homozygous mutant thymocytes, indicating that exon 5 was skipped in splicing events in the mutant allele. Western blot analysis of homozygous mutant thymocytes using a rabbit antiserum to the cytoplasmic carboxy terminal ten amino acids detected a small amount of a truncated protein.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations