Ikzf1^tm1Kge

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Species

Mus musculus

Symbol

Ikzf1^tm1Kge

Category

allele

Allele of gene

Ikzf1

Transgenic Constructs

None

Synonyms

• DN^-
• Ik7DN

Description

An 8.5 kb genomic fragment containing part of exon 3 and exon 4 was replaced with a neomycin cassette. The mutation deletes zinc fingers 1, 2, and 3, which mediate the high affinity binding domain of the protein. Mutant mRNA was detected by RT-PCR analysis. Low amounts of mutant protein were detected by western analysis. Gel retardation assays using nuclear extracts from spleen and thymus showed no binding activity in homozygous mutant animals. A subsequent study suggested this allele generates a dominant-negative protein.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations