Cd86^tm2Shr

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Species

Mus musculus

Symbol

Cd86^tm2Shr

Category

allele

Allele of gene

Cd86

Transgenic Constructs

None

Synonyms

• B7-1/2-
• B7-1/B7-2-

Show All 6

Description

To create a knockout of both Cd80 and Cd86 in mice, an ES cell line in which the Cd80 gene was mutated was subsequently used in a second round of gene targeting. A hygromycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences of the Cd86 gene. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations