Allele/Variant

Brca1tm2Arge

Species
Mus musculus
Symbol
Brca1tm2Arge
Category
allele
Allele of gene
Brca1
Transgenic Constructs
None
Synonyms
  • Brca1tr
Description
A loxP-flanked neomycin and thymidine kinase selection cassette was inserted into exon 11. The loxP flanked cassette was removed in ES cells by Cre mediated recombination prior to the production of chimeric mice, leaving a 50 bp insertion containing a loxP site. Northern blot analysis on RNA derived from total RNA of homozygous embryos demonstrated that aberrant transcripts were produced from this allele that either lacked exon 11, or contained exon 11 with the insertion, resulting in a frameshift mutation after the first 924 amino acids. This mutation was made to mimic a truncated protein associated with human breast cancer.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations