Kif3c^tm1Gsn

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Species

Mus musculus

Symbol

Kif3c^tm1Gsn

Category

allele

Allele of gene

Kif3c

Transgenic Constructs

None

Synonyms

• Kif3c^typeI

Description

Two loxP sites were introduced to the intron and 5' untranslated region flanking exon 1, and a third loxP site was introduced with a PGK-TK/PGK-neo cassette directly downstream exon 1 via homologous recombination. Exon 1 and the PGK-TK/PGK-neo cassette were removed by transient expression of cre recombinase in correctly targeted ES cells resulting in the deletion of 2.1 kb of DNA encoding amino acids 1-518 which include the whole motor region and half of the alpha-helical coiled coil domains. Northern blot analysis detected a truncated transcript encoding the tail end of the gene in homozygous mutant animals. Truncated protein product was detected in homozygous mutants by Western blot analysis using antibodies recognizing the tail end of the protein. Immunoprecipitation of brain lysates from homozygous mutants demonstrated the ability of the truncated protein to specifically bind Kif3a and not Kif3b.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations