Allele/Variant

Gfaptm4Ito

Species
Mus musculus
Symbol
Gfaptm4Ito
Category
allele
Allele of gene
Gfap
Transgenic Constructs
None
Synonyms
  • Gfaphm3
Description
The 5' portion of exon 1 was replaced with corresponding human sequence mutagenized to contain substitution mutations at all 5 phosphorylation sites, Thr7, Ser8, Ser13, Ser17, and Ser38 (human positions). The incorporation of the specific mutations was verified by sequence analysis as well as by Western blot analysis using antibodies directed toward the phosphorylation sites. Northern blot analysis of total brain RNA showed that transcript levels were unaffected in mutant mice. Immunohistochemical analysis of brain tissue showed that while the distribution of protein was conserved in mutant mice, protein levels were reduced in various regions.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations