Esr1^tm2Ksk

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Species

Mus musculus

Symbol

Esr1^tm2Ksk

Category

allele

Allele of gene

Esr1

Transgenic Constructs

None

Synonyms

None

Description

Missense mutations (leucine to alanine) of the first two codons encoding hydrophobic residues were introduced in exon 9, which encodes the AF-2 motif. Previous in vitro studies showed that the disruption of these residues conferred insensitivity to estradiol. Sequence encoding a carboxy terminal histidine epitope tag was also added to allow for detection of the mutant protein. A floxed neo cassette that was inserted into the 3' untranslated region was excised from ES cells via expression of cre recombinase. RT-PCR analysis of RNA isolated from ES cells showed greater amounts of transcript after the expression of cre than prior to it, indicating that presence of neo affected mRNA stability.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations