Runx1^tm3Dow

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Species

Mus musculus

Symbol

Runx1^tm3Dow

Category

allele

Allele of gene

Runx1

Transgenic Constructs

None

Synonyms

• Aml^LSL-A/E
• AML1-ETO knockin

Show All 5

Description

A floxed stop cassette containing a strong polyA signal was inserted into intron 3, upstream of the murine/human hybrid sequence engineered from human t(8;21) cDNA and used to generate Runx1^tm2Dow. While trace levels of an alternatively spliced transcript including the downstream region were detected, no such protein was identified in mutant mice by Western blot analysis. Excision of the floxed polyA signal and the resultant expression of the inserted human sequence was achieved when mice carrying this allele were bred with transgenic mice expressing cre recombinase.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations