Polk^tm1.1Rsky

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Species

Mus musculus

Symbol

Polk^tm1.1Rsky

Category

allele

Allele of gene

Polk

Transgenic Constructs

None

Synonyms

• Polk^tm1.1Esp

Description

A loxP-flanked exon 6 and downstream floxed neo cassette were removed by crossing chimeric animals to a cre deleter strain resulting in the deletion of essential catalytic residues apartate 197 and glutamate 198, as well as a frameshift mutation via splicing from exon 5 to exon 7. RT-PCR analysis of testis RNA confirmed the presence of a shorter transcript in mutant animals. Northern blot analysis showed that the mutant transcript is expressed at 5 times less than the wild-type allele.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations