The mouse model of Liddle's syndrome was created by deleting the loxP-flanked neomycin resistance cassette following the engineered stop codon at position 566. This was achieved by mating Scnn1btm1Ipt animals to cre-expressing line TgN(EIIa-cre)C5379Lmgd. Northern blot analysis of colon and kidney mRNA showed a significant decrease in gene expression in mutant animals.