Allele/Variant

Tg(Cd8b1)46Erob

Species
Mus musculus
Symbol
Tg(Cd8b1)46Erob
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • dnlyt3
Description
Site directed mutagenesis was used to change the Arg-181 of a Cd8b1 gene fragment to a stop codon. This fragment replaced the corresponding sequence in a genomic fragment of the Cd8b1 gene. A 5 kb fragment with the human CD2 locus-controlling region downstream of the mutated gene was ligated to the genomic clone and the entire construct was injected into embryos. A founder with ~20 copies/diploid genome, designated dnlyt3, was used for analysis. "CD8b1" differs from the "CD8b2" allele normally expressed in the C57BL/6 mouse strain. Monoclonal antibodies can distinguish the endogenous protein from the transgene product in hemizygous transgenic mice.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
Knock-down Targets
None
Regulatory Regions
None
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations