Gab1^tm3Wbm

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Species

Mus musculus

Symbol

Gab1^tm3Wbm

Category

allele

Allele of gene

Gab1

Transgenic Constructs

None

Synonyms

• Gab1deltaGrb2 (delta 341-348/L524P)

Description

A cDNA encoding amino acids 198-695 with a deletion of amino acids 341 to 348, an amino acid substitution of leucine for proline at position 542 (L542P) and a floxed neo cassette were knocked into exon d. The neo cassette was removed by cre-mediated recombination following a cross to a cre deleter strain (Tg(CMV-cre)1Cgn). The modification to the coding region is thought to interfere with protein-protein interactions with Grb2. Expression of the mutant protein confirmed by western blot analysis on protein extracts from E12 embryos was reduced by 40% to 50% relative to levels in wild-type mice.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations