Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Epm2aTg(TcraK,TcrbK)TG-BFlv
- Species
- Mus musculus
- Symbol
- Epm2aTg(TcraK,TcrbK)TG-BFlv
- Category
- allele
- Allele of gene
- Epm2a
- Transgenic Constructs
- None
- Synonyms
- H2-Kk
- TG-B
- Description
- The transgenic mice express rearranged Valpha and Vbeta genes from a CTL clone that recognized the SV40 large T antigen (SV-T). The V and J regions of a transgene construct (made in J:107523) were replaced with the V and J regions of the clone K T cell receptor. The synthetic alpha and beta constructs were coinjected into 1 day embryos. Three lines were obtained and line TG-B was further characterized. The transgene in line TG-B inserted into intron 1. Western blot analysis confirmed reduced protein expression.
- Additional Information
- Literature