Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Gt(ROSA)26Sortm1(HBEGF)Awai
- Species
- Mus musculus
- Symbol
- Gt(ROSA)26Sortm1(HBEGF)Awai
- Category
- allele
- Allele of gene
- Gt(ROSA)26Sor
- Transgenic Constructs
- Synonyms
- Gt(ROSA)26Sortm1(Dtr)ThBu
- iDTR
- Description
- A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- HBEGF
- Knock-down Targets
- None
- Regulatory Regions
- None