Allele/Variant

Relatm1.2Gho

Species
Mus musculus
Symbol
Relatm1.2Gho
Category
allele
Allele of gene
Rela
Transgenic Constructs
None
Synonyms
  • p65-R274A, S276A(RAPA)
Description
By homologous recombination in ES cells, endogenous DNA sequence for arginine 274 and serine 276 residues in exon 7 of Rela gene were replaced with codons for alanine at both locations followed by a neo cassette flanked by two loxP sites inserted into intron after Exon 7. A neo cassette was then deleted either in the recombined ES cells by transfection of cre-recombinase or in mice by crossing with splicer mice harboring a Cre transgene, leaving only single loxP sites. The presence of the mutation was confirmed by RT-PCR and DNA sequencing. Somewhat reduced amount mutant protein was translated.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations