Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Tg(Itgax-TGFBR2)1Flv
- Species
- Mus musculus
- Symbol
- Tg(Itgax-TGFBR2)1Flv
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- CD11c-dnTGFBRII
- CD11cdnR
- Description
- The human TGFBRIII (Tgfbr2 transforming growth factor, beta receptor II) gene sequence between nucleotides -7 to +573 encoding the extracellular and transmembrane regions of TGFBR2 (dominant negative, dnTGFBR2) was inserted into exon 3 of the rabbit beta globin gene in the plasmid Itgax promoter vector pDOI-5. The construct was injected into (C57BL/6 x C3H)F1 hybrid fertilized eggs. RT-PCR confirmed expression of dnTGFBR2 in Itgax (CD11c)-expressing cells including subsets of natural killer cell (NK) and dendritic cell (DC) subsets. No expression is detected in NKT cells, T cells or B cells. Expression is sufficient to block TFG beta signaling through the functional receptor exclusively in DCs and NK cells.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- Knock-down Targets
- None
- Regulatory Regions
- None