The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2445bp between positions 4612057-4614501 of Chromosome 19 (Genome Build37). All coding exons (exons 2 and 3) were replaced with a beta-geo cassette (beta-galactosidase/neomycin fusion). The encoded protein was undetectable in immunoblots of whole-brain lysates derived from homozygous mutant mice.