Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
- Species
- Mus musculus
- Symbol
- Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
- Category
- allele
- Allele of gene
- Gt(ROSA)26Sor
- Transgenic Constructs
- None
- Synonyms
- Ai9
- Ai9(RCL-tdT
- Description
- The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
- Additional Information
- Literature