Allele/Variant

Selltm2.1Tft

Species
Mus musculus
Symbol
Selltm2.1Tft
Category
allele
Allele of gene
Sell
Transgenic Constructs
None
Synonyms
  • L(E)
Description
The membrane-proximal region was replaced with DNA encoding a floxed neomycin resistance gene and the 7 amino acid (APVVPTR) membrane-proximal region of mouse E-selectin. It was predicted that this mutation would prevent cleavage of the protein upon cellular activation. Because the presence of the neo resistance gene was found to reduce expression of the gene locus in Selltm2Tft homozygotes, the neo gene was removed by breeding with Cre recombinase transgenic mice. Cell surface levels of mutated protein in homozygote blood, spleen, and lymphocytes was 168%, 214%, and 178%, respectively, of wild-type controls as determined by flow cytometry. Mutated protein was not cleaved from the cell surface upon cellular activation of lymphocytes.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations