Rap1a^tm1Tze

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Species

Mus musculus

Symbol

Rap1a^tm1Tze

Category

allele

Allele of gene

Rap1a

Transgenic Constructs

None

Synonyms

None

Description

A targeting construct was made by replacing a 420-bp region encoding the N-terminal 19 amino acids, which include conserved residues and part of the GTP-binding motif, with an internal ribosome entry site (IRES)/LacZ/ neomycin cassette. Shortened mRNAs for the locus were detected in tissue of homozygous mice. These resulted from an alternative splicing site 5' of the neomycin cassette and an alternative ATG start site that would result in a 51 amino acid truncated protein. However, no protein was detected in immunoblot analysis of thymus and spleen extracts so the authors concluded this was a null allele. LacZ staining was evident in E12.5 embryos.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations