Allele/Variant

Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli

Species
Mus musculus
Symbol
Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • tet-O-CMV-IFNgamma-hGH
  • TG CC10-rtTA-IFNgamma
Description
The CC10-rtTA-hGH construct contains the CC10 promoter, the reverse tetracycline transactivator (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The tet-O-CMV-IFNgamma-hGH construct contains a polymeric tetracycline operator (tet-O), minimal CMV promoter, murine IFNgamma cDNA, and hGH intronic and polyadenylation signals. Both constructs were coinjected into fertilized oocytes, and dual positive founder animals were obtained. In the presence of doxycycline (dox), rtTA is able to bind in trans to the tet-O, and the VP-16 transactivator activates LIF gene transcription. In the absence of dox, rtTA binding occurs at very low levels, and only low level gene transcription is noted. Transgenic lines 15 and 21 were also created.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
Knock-down Targets
None
Regulatory Regions
None
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

Phenotype
Annotation details
Source
References
abnormal lung volumeMGI
emphysemaMGI
increased apoptosisMGI
increased lung complianceMGI
increased lymphocyte cell numberMGI
increased macrophage cell numberMGI
increased neutrophil cell numberMGI
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Disease Associations

No data available