Functional VDJ beta (V beta 11 and J beta 2.4) and VJ alpha (V alpha x4.1 and J alpha 33) rearrangements were isolated from the beta cell cytotoxic CD4(+) T cell clone NY4.1. Upstream regulatory sequences together with either TCR-beta or TCR-alpha enhancers, as appropriate, were associated with VDJbeta or VJalpha. The resulting TCR alpha and beta constructs were coinjected and cointegrated into (SJLXC57BL/6J)F2 oocytes.