Allele/Variant

Kmt2atm1Brad

Species
Mus musculus
Symbol
Kmt2atm1Brad
Category
allele
Allele of gene
Kmt2a
Transgenic Constructs
None
Synonyms
  • Mllflox
Description
A targeting vector was constructed where an upstream loxP site was cloned into a blunted NsiI site in intron 7 and a floxed neomycin resistance cassette was cloned into a BglII site in intron 9. Targeting took place in PC3 ES cells homozygous for the cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase in male germ cells. Cre mediated recombination subsequently removed only the neomycin cassette upon transmission from male chimeric mice. Offspring from the male chimeras in which exons 8 and 9 remained floxed were selected for further breeding. Expression was unaffected as determined by immunoblot analysis of splenic extracts.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations