Gucy1a1^tm1.1Brou

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Species

Mus musculus

Symbol

Gucy1a1^tm1.1Brou

Category

allele

Allele of gene

Gucy1a1

Transgenic Constructs

None

Synonyms

• sGCalph1^-

Description

Exon 6 of Gucy1a3 gene which codes for a conserved portion of the catalytic domain was flanked by a loxP and a loxP-neo-loxP cassette. The exon 6 and neo cassette was removed in ES cells by transient cre expression leaving a single loxP in place. Northern blot and RT-PCR confirmed excision of exon 6 in the transcript; however, immunoblot revealed a smaller mutant 59-kDa protein in homozygous mice. Mutant protein was determined to be inactive, thus it is functional null allele.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations