Allele/Variant

Gt(ROSA)26Sortm1(CAG-taulacZ)Bene

Species
Mus musculus
Symbol
Gt(ROSA)26Sortm1(CAG-taulacZ)Bene
Category
allele
Allele of gene
Gt(ROSA)26Sor
Transgenic Constructs
None
Synonyms
  • Gt(ROSA)26Sortm1(CAG-MAPT/lacZ)Bene
  • Gt(ROSA)26Sortm1(CAG-taulacZ)Bene
Description
A genetic construct that reports cre recombinase activity with tauLacZ reporter gene was inserted in reverse orientation into the Gt(ROSA)26Sor safe harbor locus. The reporter construct included a CAG promoter, a loxP flanked stop cassette composed of neomycin resistance gene and multiple polyadenylation sequences, a tauLacZ reporter gene, and a polyadenylation sequence (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA). The construct was electroporated into B6(Cg)-Tyrc-2J derived CY2.4 embryonic stem cells. Southern blot positive embryonic stem cell clones were injected into recipient C57BL/6J blastocysts. Positive male chimeras were bred with B6(Cg)-Tyrc-2J females. Albino pups were genotyped by PCR, then bred to B6J mice.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations