Allele/Variant

Chat/Slc18a3tm1.1Vpra

Species
Mus musculus
Symbol
Chat/Slc18a3tm1.1Vpra
Category
allele
Allele of gene
Slc18a3
Transgenic Constructs
None
Synonyms
  • VAChTdel
Description
One loxP sequence was added 260 bp upstream from the translational initiation codon of Slc18a3 (within the first intron of Chat), and a second loxP was added approximately 1.5 kb downstream from the stop codon. This flanking region included the second exon of Chat. The TK-Neo cassette was added immediately after the second loxP and was followed by a third LoxP (all in the same direction). The floxed region (including the TK-Neo cassette) was excised from mice by in vivo cre mediated recombination. No Slc18a3 mRNA was detected in homozygous mouse brain by qPCR. Slc18a3 protein was undetected in homozygous mutant mice by Western blot analysis of spinal cord extracts and immunostaining of the neuromuscular junction. Chat protein level was increased in E18.5 spinal cords and Chat mRNA level was increased as detected by qPCR from E18.5 mouse brains.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

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    Disease Associations

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