Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Tg(BEST1-rtTA,tetO-cre)1Yzl
- Species
- Mus musculus
- Symbol
- Tg(BEST1-rtTA,tetO-cre)1Yzl
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- PVMD2-rtTA::tetO-PhCMVcre
- VMD2-cre
- Description
- Two transgenic fragments were co-injected to generate the mouse line. One fragment contains a 3 kb human BEST1 promoter fragment driving expression of the tetracycline-inducible transactivator rtTA and the SV40 polyA sequence. The second fragment consists of the tetracycline-responsive regulatory element (tetO) and human cytomegalovirus immediate early enhancer (hCMV) controlling expression of a translationally optimized cre sequence followed by the mouse metallothionein (Mt1) polyadenylation signal. After co-injection into zygotes, 10 founders were obtained with co-integration of both fragments into a single chromosome being observed in all founders. One line demonstrating substantial activity in Muller glial cells was characterized further.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- None
- Knock-down Targets
- None
- Regulatory Regions