The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 1011bp between positions 55619824-55620834 of Chromosome 13 (Build GRCm39). The entire coding region was replaced by a cassette containing the lacZ gene expressed under control of the endogenous promoter and an independently expressed neomycin resistance gene. Immunoblotting of brain lysates confirmed the absence of protein expression in homozygous mutant mice.